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Project Topic:
ISOLATION AND IDENTIFICATION OF MICROORGANISM IN STREAM WATER [ATOLAGBE] IREE TOWN
Project Information:
Project Body:
ABSTRACT
The stream water was examined for microorganism present in it. The isolated and identified bacteria are Bacillus cereus and Staphylococcus aureus. While fungi are Aspergilllus niger and Sacchromyces cerevisae Pnicillium chrysogenum.
TABLE OF CONTENT
TITLE PAGE I
CERTIFICATION II
DEDICATION III
ACKNOWLEDGMENT IV
TABLE OF CONTENT V
ABSTRACT VI
CHAPTER ONE
1.0 INTRODUCTION 1 – 2
CHAPTER TWO
2.0 LITERATURE REVIEW 3 – 6
2.1 PURPOSE OF STUDY 6
CHAPTER THREE
3.0 MATERIALS AND METHODS 7
3.1 STERILIZATION OF MATERIALS 7
3.2 COLLECTION OF SAMPLE 7
3.3 PREPARATION OF MEDIA 7 – 8
3.4 SERIAL DILUTION 8
3.5 INOCULATION OF AGAR 8
3.6 SUB-CULTURING OF MICROBIAL GROWTH 8
3.7 MAINTENANCE OF PURE CULTURE 8
3.8 CHARACTERIZATION OF FUNGAL ISOLATES 9
3.9 CHARACTERIZATION OF BACTERIAL ISOLATES 9
3.9.0 GRAM STAINING 9
3.9.1 SPORE STAINING 10
3.9.2 COAGULASE TEST 10
3.9.3 OXIDASE TEST 10
3.9.4 CATALASE TEST 10
3.9.5 MOTILITY TEST 10 – 11
3.9.6 IDENTIFICATION OF FUNGAL ISOLATES 11
3.9.7 IDENTIFICATION OF BACTERIAL ISOLATES 11
CHAPTER FOUR
4.0 RESULTS AND DISCUSSION 12 – 15
CHAPTER FIVE
5.0 CONCLUSION AND RECOMMENDATION 16 – 17
REFERENCES 18